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Fig. 5 A negative effect of LAR deletion in the reporter gene expression from the LWS1/GFP-LWS2/RFP-PAC clones.
(A) Schematic representation of the construction of ”LAR-LWS1/GFP-LWS2/RFP-PAC(E) and (H). (B, C, D) Images of a whole mount retina of an adult Tg(LWS1/GFP-LWS2/RFP-PAC(H))#430 (B), Tg(”LAR-LWS1/GFP-LWS2/RFP-PAC(E))#1143 (C), and Tg(”LAR-LWS1/GFP-LWS2/RFP-PAC(H))#1107 (D) fish. The dorsal side is oriented at the top of each panel, and the nasal side is on the left. The GFP signals appear as green and the RFP signals appear as orange. The right panel is the overlay of the left and center panels. (E) A vertical and expanded view of the photoreceptor layer from the same transgenic line as shown in (D). The LDCs were immunostained with the antibody against the zebrafish red opsin (magenta). The GFP signals appear as green. Overlap of the two signals appears as white. Scale bars = 10 μm.