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Fig. 4 llb mutation causes the null phenotypes of isl1. A–D: Primary sensory neurons of the embryos injected with 2 μg/μl of isl1 AMO (A, B) and 2 μg/μl of isl1 control AMO (C, D) at 28 hpf. Tg neurons (A, C) and RB neurons (B, D) were stained by anti-acetylated α-tubulin antibody. E, F: The peripheral axons of RB neurons in the embryos injected with 2 μg/μl of isl1 AMO and 28 ng/μl of isl1 mRNA (E), or 28 ng/μl of isl1 mRNA, which has the llb mutation (F), at 28 hpf. All panels are lateral views with anterior at left. G, H: Quantitative data for the rescue experiments. Data are mean ± SEM; n = 10; *P < 0.05, different from the control embryos using 2-tailed paired Student′s t-test.