PUBLICATION

Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons

Authors

Tanaka, H., Nojima, Y., Shoji, W., Sato, M., Nakayama, R., Ohshima, T., and Okamoto, H.

ID

ZDB-PUB-101201-20

Date

2011

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 240(1): 9-22 (Journal)

Registered Authors

Nakayama, Ryoko, Okamoto, Hitoshi, Sato, Miki, Shoji, Wataru, Tanaka, Hideomi

Keywords

Islet1, Dpysl3, CRMP4, semaphorin, Rohon-Beard neuron, axon guidance, zebrafish

MeSH Terms

Amino Acid Sequence
Animals
Animals, Genetically Modified
Axons/metabolism
Axons/physiology*
Base Sequence
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Genetic Loci
Homeodomain Proteins/genetics
Homeodomain Proteins/metabolism
Homeodomain Proteins/physiology*
LIM-Homeodomain Proteins
Models, Biological
Molecular Sequence Data
Motor Neurons/metabolism
Motor Neurons/physiology
Mutation/physiology
Nerve Tissue Proteins/genetics
Nerve Tissue Proteins/metabolism
Nerve Tissue Proteins/physiology*
Sensory Receptor Cells/metabolism
Sensory Receptor Cells/physiology*
Sequence Homology
Spinal Nerves/abnormalities
Spinal Nerves/embryology
Spinal Nerves/metabolism
Transcription Factors
Zebrafish/embryology
Zebrafish/genetics
Zebrafish/physiology
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Zebrafish Proteins/physiology*

(all 35)

PubMed

21108318 Full text @ Dev. Dyn.

Abstract

We isolated a novel zebrafish mutant, lullaby (llb), and showed that the llb locus encodes the zebrafish orthologue of isl1. Rohon-Beard (RB) primary sensory neurons are multipolar neurons that extend their central axons longitudinally within the spinal cord and also extend their peripheral axons under the skin. In llb embryos, the outgrowth of the peripheral axons of RB neurons was selectively impaired, which correlated with down-regulation of the expression of dihydropyrimidinase-like 3 (dpysl3, also known as collapsin response mediator protein 4, crmp4). Antisense morpholino oligonucleotide (AMO)-mediated knockdown of dpysl3 inhibited the outgrowth of the peripheral axons of RB neurons, and semaphorin 3d (sema3d) AMO enhanced this effect. These data indicate that Dpysl3 is cooperating with Sema3d in the peripheral axon outgrowth, and Isl1 is required for the selective outgrowth of the peripheral axons of RB neurons by maintaining the expression of dpysl3.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
dpysl3	isl1a^rw436/rw436	standard conditions	Prim-5	Not Detected	Rohon-Beard neuron	ISH	Fig. 5
dpysl3	isl1a^rw436/rw436	standard conditions	Prim-5		trigeminal ganglion	ISH	Fig. 5
dpysl3	WT	standard conditions	Prim-5		Rohon-Beard neuron	ISH	Fig. 5
dpysl3	WT	standard conditions	Prim-5		trigeminal ganglion	ISH	Fig. 5
GFP	rw011aTg	standard conditions	Prim-5		Rohon-Beard neuron	IFL	Fig. 7
isl1a	isl1a^rw436/rw436	standard conditions	20-25 somites	Not Detected	primary motor neuron	ISH	Fig. 2
isl1a	isl1a^rw436/rw436	standard conditions	Prim-5	Not Detected	primary motor neuron	ISH	Fig. 2
isl1a	isl1a^rw436/rw436	standard conditions	Prim-5	Not Detected	Rohon-Beard neuron	ISH	Fig. 2
isl1a	isl1a^rw436/rw436	standard conditions	20-25 somites	Not Detected	Rohon-Beard neuron	ISH	Fig. 2
isl1a	isl1a^rw436/rw436	standard conditions	Prim-5		trigeminal ganglion	ISH	Fig. 5

1 - 10 of 29

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-dpysl3	standard conditions	Prim-5	peripheral nervous system neuron axonogenesis decreased occurrence, abnormal	Fig. 9
WT + MO1-dpysl3	standard conditions	Prim-5	peripheral nervous system neuron axonogenesis decreased occurrence, abnormal	Fig. 6
WT + MO1-dpysl3	standard conditions	Prim-5	Rohon-Beard neuron axon decreased amount, abnormal	Fig. 9
WT + MO1-dpysl3	standard conditions	Prim-5	Rohon-Beard neuron axon decreased amount, abnormal	Fig. 6
WT + MO1-dpysl3 + MO2-sema3d	standard conditions	Prim-5	peripheral nervous system neuron axonogenesis decreased occurrence, abnormal	Fig. 9
WT + MO1-dpysl3 + MO2-sema3d	standard conditions	Prim-5	Rohon-Beard neuron axon decreased amount, abnormal	Fig. 9
WT + MO2-sema3d	standard conditions	Prim-5	peripheral nervous system neuron axonogenesis decreased occurrence, abnormal	Fig. 9
WT + MO2-sema3d	standard conditions	Prim-5	peripheral nervous system neuron axonogenesis decreased occurrence, abnormal	Fig. 6
WT + MO2-sema3d	standard conditions	Prim-5	Rohon-Beard neuron axon decreased amount, abnormal	Fig. 9
WT + MO2-sema3d	standard conditions	Prim-5	Rohon-Beard neuron axon decreased amount, abnormal	Fig. 6

1 - 10 of 31

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
rw011aTg	Tg(isl1a-hsp70l:GFP)	Transgenic Insertion
rw436		Point Mutation	isl1a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
dpysl3	MO1-dpysl3	MRPHLNO
isl1a	MO4-isl1a	MRPHLNO
sema3d	MO2-sema3d	MRPHLNO

Fish

Fish
isl1a^rw436/rw436
rw011aTg
WT
WT + MO1-dpysl3
WT + MO1-dpysl3 + MO2-sema3d
WT + MO2-sema3d
WT + MO4-isl1a

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-cntn2	monoclonal	cntn2		Mouse
Ab2-gad	monoclonal		IgG1	Mouse
zn-1	monoclonal		IgG1	Mouse
zn-5	monoclonal	alcama	IgG1	Mouse
znp-1	monoclonal	syt2b	IgG2a	Mouse

1 - 5 of 5

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Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Entity Type	Entity Symbol	Location
	rw436	Chr: 5 Details
SSLP	z6094	Chr: 5 Details
SSLP	z9871	Chr: 5 Details

Tanaka et al., 2011 ZDB-PUB-101201-20 PMID:21108318

Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons

Tanaka et al., 2011

ZDB-PUB-101201-20
PMID:21108318