|
Fig. 5 irx2a expression signal is disrupted in irx1a morphant and ath5 morphant retinas. Expression pattern of irx2a and irx4a in irx1a MO2 morphant retinas (G–I and J–L) and ath5 morphant retinas (M–O and P–R) were revealed by in situ hybridization of 30-, 48-, and 72-hpf embryos. In irx1a morphant retina, irx2a expression was completely absent at all the examined developmental stages (G–I), while the expression of irx4a was slightly weaker than the control morphants (J–L). Similar results were observed in ath5 morphant retina; while irx2a expression was disrupted (M–O), similar irx4a expression was detected in the control morphant retina (P–R).