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Fig. S15 Simultaneous visualization of O-glycans and sialylated glycans at 24 hpf. Zebrafish embryos were microinjected with GalNAz or no sugar, allowed to develop to 24 hpf, and then bathed in NaIO4 (500 μM, 30 min) or no reagent. Embryos were then reacted in a mixture of DIFO-555 (100 μM) and AO-488 (100 μM) in PBS (pH 6.7) for 1 h and imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images and corresponding brightfield images. Green, AO-488; red, DIFO-555. Scale bars: 200 μm (A), 20 μm (B).
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Full text @ Proc. Natl. Acad. Sci. USA