ZFIN Image: Chi et al., 2010, Fig. 4

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Figure Caption

Fig. 4

dco Cardiac phenotype is rescued by RNA injection of Cx46 orthologs as well as by myocardial specific expression of zebrafish cx46. (A and B) Myocardial specific expression of WT [Tg(cmlc2:cx46-GFP^s882)] and mutant [Tg(cmlc2:cx46mt-GFP^s925)] zebrafish Cx46-GFP fusion proteins lead to cell membrane enrichment. Outer curvature (OC), inner curvature (IC), and atrioventricular canal (AV) are outlined by dashed lines. Atr, atrium; Ven, ventricle. (C) Injection of WT zebrafish (WT zfcx46) or mouse Cx46 (mouseCx46) mRNA as well as myocardial specific expression of WT zebrafish Cx46-GFP fusion proteins [Tg(cmlc2:cx46-GFP^s882)] rescued the dco cardiac phenotype. On the other hand, injection of zebrafish cx46^s226 (MT zfcx46) or cx43 (zfcx43) mRNA as well as myocardial specific expression of zebrafish Cx46^s226-GFP [Tg(cmlc2:cx46mt-GFP^s925)] failed to rescue the dco cardiac phenotype. (D) However, injection of zebrafish cx46^s226 mRNA or myocardial specific expression of zebrafish Cx46^s226-GFP [Tg(cmlc2:cx46mt-GFP^s925)] in WT embryos resulted in dco cardiac phenotypes. In addition, RNA overexpression of WT murine Cx46 caused a dco cardiac phenotype in a small percentage of WT embryos.

Acknowledgments

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