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Fig. S3 Eve1 regulates BMP expression but not BMP signaling. Lateral views (where discernible, dorsal to the right) of zebrafish embryos fixed for in situ staining at 80% epiboly. Genes analyzed are indicated at the left of the rows, and injections are indicated at the top of the columns. Induction of the neural markers sox3 (B) and hoxb1b (F) and suppression of bmp4 (J) by eve1 is antagonized even by low doses (7 pg/nL) of bmp2b coinjected with eve1 [100%; n = 25 (C), 100%, n = 34 (G), and 100%, n = 21 (K) respectively] (compare to A, E, and I). (D, H, and L) Expression of sox3 (D), hoxb1b (H), or bmp4 (L) following injection of low doses (7 pg/nL) of bmp2b.