PUBLICATION

Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos

Authors

Cruz, C., Maegawa, S., Weinberg, E.S., Wilson, S.W., Dawid, I.B., and Kudoh, T.

ID

ZDB-PUB-100211-28

Date

2010

Source

Proceedings of the National Academy of Sciences of the United States of America 107(8): 3564-3569 (Journal)

Registered Authors

Dawid, Igor B., Kudoh, Tetsuhiro, Maegawa, Shingo, Weinberg, Eric

Keywords

bone morphogenic protein, neural induction, organizer, posterior development, retinoic acid

MeSH Terms

Bone Morphogenetic Proteins/biosynthesis
Gene Expression Regulation, Developmental*
Tail/embryology*
Genes, Homeobox
Body Patterning/genetics*
Ectoderm/embryology*
Ectoderm/metabolism
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism
Animals
Neural Tube/embryology*
Neural Tube/metabolism
Homeodomain Proteins/biosynthesis
Embryo, Nonmammalian/metabolism
Tretinoin/metabolism
Gastrula/embryology
Gastrula/metabolism
Repressor Proteins/genetics
Repressor Proteins/metabolism*

(all 20)

PubMed

20142486 Full text @ Proc. Natl. Acad. Sci. USA

Abstract

In vertebrates, Evx homeodomain transcription factor-encoding genes are expressed in the posterior region during embryonic development, and overexpression experiments have revealed roles in tail development in fish and frogs. We analyzed the molecular mechanisms of posterior neural development and axis formation regulated by eve1. We show that eve1 is involved in establishing trunk and tail neural ectoderm by two independent mechanisms: First, eve1 posteriorizes neural ectoderm via induction of aldh1a2, which encodes an enzyme that synthesizes retinoic acid; second, eve1 is involved in neural induction in the posterior ectoderm by attenuating BMP expression. Further, eve1 can restore trunk neural tube formation in the organizer-deficient ichabod(-/-) mutant. We conclude that eve1 is crucial for the organization of the antero-posterior and dorso-ventral axis in the gastrula ectoderm and also has trunk- and tail-promoting activity.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
aldh1a2	WT	control	75%-epiboly		presumptive paraxial mesoderm	ISH	Fig. 3
aldh1a2	WT	control	75%-epiboly		presumptive paraxial mesoderm	ISH	Fig. 2
aldh1a2	WT + MO1-eve1	standard conditions	75%-epiboly	Not Detected	presumptive paraxial mesoderm	ISH	Fig. 2
bmp2b	WT	control	75%-epiboly		ectoderm	ISH	Fig. 2
bmp2b	WT	control	75%-epiboly		ectoderm	ISH	Fig. 4
bmp2b	WT	control	75%-epiboly		margin	ISH	Fig. 4
bmp2b	WT	control	75%-epiboly		margin	ISH	Fig. 2
bmp2b	WT + MO1-eve1	standard conditions	75%-epiboly		ectoderm	ISH	Fig. 2
bmp2b	WT + MO1-eve1	standard conditions	75%-epiboly		margin	ISH	Fig. 2
bmp4	WT	control	Shield		unspecified	ISH	Fig. 2

1 - 10 of 44

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
ctnnb2^p1/p1	standard conditions	Prim-5	head aplastic, abnormal	Fig. 5
ctnnb2^p1/p1	standard conditions	Prim-5	trunk morphology, abnormal	Fig. 5
ctnnb2^p1/p1	standard conditions	Prim-5	whole organism wholly ventralized, abnormal	Fig. 5

1 - 3 of 3

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
p1		Unknown	ctnnb2

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
aldh1a2	MO5-aldh1a2	MRPHLNO
bmp2b	MO4-bmp2b	MRPHLNO
eve1	MO1-eve1	MRPHLNO

Fish

Fish
ctnnb2^p1/p1
WT
WT + MO1-eve1
WT + MO4-bmp2b
WT + MO5-aldh1a2

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Cruz et al., 2010 ZDB-PUB-100211-28 PMID:20142486

Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos

Cruz et al., 2010

ZDB-PUB-100211-28
PMID:20142486