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Fig. S5 PDGF signaling is required for DNA synthesis in 4-dpa epicardial cultures. (A) Dose-dependent effect of PDGFR inhibitor on DNA synthesis as percentage of BrdU-positive cells. Error bars indicate SEM. *P < 0.05. (B) Monolayer of epicardial cells migrated out from the apex of 4-dpa regenerating hearts after 3 d in culture. BrdU was added to the culture at the same time as DMSO (n = 12 hearts), PDGFR inhibitor (2 μM, n = 11 hearts), ROCK inhibitor (2 μM, n = 9 hearts), or PI3K inhibitor (inh; 2 μM, n = 13 hearts) for 1 d (i.e., from day 3 to day 4 in culture). BrdU staining is shown in red, and DAPI staining is shown in blue. (Scale bar = 50 μm.) (C) DNA synthesis quantified as the percentage of BrdU-positive cells. Error bars indicate SEM. *P < 0.01.