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Fig. S3 Primary epicardial cell culture in vitro from sham-operated heart explants. Explant cultures of sham-operated hearts (n = 12 hearts) were cultured for 3 d on fibrin gels and then fixed and stained (actin, green; DAPI, blue; epicardin, red; ZO1, red). Actin is localized at the subcortical region. Each individual explant was imaged in at least three different fields of view; a representative image is shown. (Scale bar = 50 μm.)
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