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Fig. 5 Expression of vascular markers in Notch mutant embryos is undisturbed at 24 hpf. Expression of Flk1 (A-D) in the zebrafish trunk reveals expression in vessels in control (A) and the aei, bea, and des Notch mutant embryos (B-D), even in the disrupted posterior somites (between black arrowheads). The anterior somites are normal (black asterisk). The disorganised ISVs are shown at higher magnification, inset. Expression of Efnb2 an arterial marker (E) is also present in each of the mutants (F-H), but in an irregular pattern in posterior somites (between black arrowheads; shown at higher magnification, inset). Efnb2 is expressed in a normal pattern in anterior somites of each mutant (white arrows, inset; F-H). Scale bar = 200 μm.