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Fig. S1A Specific blocked of GFP reporter expression by cam-kii inhibitors MO. A: Two GFP reporter plasmids (cam-kiin1: gfp and cam-kiin2: gfp) that encompassed the MO target sequences were constructed. Embryos that received 100 pg cam-kiin1: gfp or cam-kiin2: gfp resulted in mosaic GFP expression at 24 HPF. Co-injection of cam-kiin1: gfp or cam-kiin2: gfp with corresponding MO significantly diminished GFP expression. Left panel in each group: the gross morphology was pictured under light contrast microscopy. Right panel in each group: the expression of GFP was detected under fluorescent microscopy. B: Cell lysates derived from embryos injected with cam-kiin1: gfp (lane 1), cam-kiin1: gfp + cam-kiin1 MO (lane 2), cam-kiin2: gfp (lane 3), and cam-kiin2: gfp + cam-kiin2 MO (lane 4) were subjected to Western blot analysis using anti-GFP antibody. Significantly decreased GFP expression was detected in lane 2 and 4. β-actin served as internal control.