PUBLICATION
Zebrafish calcium/calmodulin-dependent protein kinase II (cam-kii) inhibitors: Expression patterns and their roles in zebrafish brain development
- Authors
- Hsu, L.S., and Tseng, C.Y.
- ID
- ZDB-PUB-101011-53
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(11): 3098-3105 (Journal)
- Registered Authors
- Keywords
- brain, cam-kii inhibitor, expression pattern, zebrafish
- MeSH Terms
-
- Animals
- Brain/embryology*
- Brain/metabolism*
- Calcium-Calmodulin-Dependent Protein Kinase Type 2/antagonists & inhibitors*
- Immunoprecipitation
- In Situ Hybridization
- Prosencephalon/embryology
- Prosencephalon/metabolism
- Protein Kinase Inhibitors/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Rhombencephalon/embryology
- Rhombencephalon/metabolism
- Telencephalon/embryology
- Telencephalon/metabolism
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 20925123 Full text @ Dev. Dyn.
Citation
Hsu, L.S., and Tseng, C.Y. (2010) Zebrafish calcium/calmodulin-dependent protein kinase II (cam-kii) inhibitors: Expression patterns and their roles in zebrafish brain development. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(11):3098-3105.
Abstract
Calcium/calmodulin-dependent protein kinase II (CaM-KII) plays a critical role in neuronal functions. In this report, we demonstrate the expression patterns, functional analysis, and development role of the two zebrafish CaM-KII inhibitors, cam-kiin1 and cam-kiin2. Both of these genes were detected in the 5-somite stage and are persistently expressed thereafter. The RNA transcripts of cam-kiin1 were prominently expressed in the forebrain and hindbrain regions, especially in the telencephalon, while cam-kiin2 was detected in the anterior brain region and neurons of the hindbrain. Through GST-pull down, co-immunoprecipitation, and kinase assay, cam-kii inhibitors can bind to and reduce cam-kiiα activity. However, no overt alternation of brain marker such as ngn1, otx2, and pax2.1 was observed in morphants received each one or combined MO. Our results suggest that the two cam-kii inhibitors exhibited distinct expression pattern and may play a minor role in zebrafish brain development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping