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Fig. S3 Characterization of Antibodies against Zebrafish CFTR (Related to Figures 2 and 3)
(A) In western blots of 120 hpf extracts the antibodies recognized a ~190KDa band. The intensity of the CFTR band was greatly reduced upon anti-sense morpholino knockdown.
(Bi) Whole mount staining of 120 hpf larvae showed a tight localization of CFTR to the apical membrane of gut enterocytes (arrow).
(Bii) In cross sections of the gut virtually all the CFTR protein was localized to the apical membrane, including the microvilli (arrow).
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