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Fig. S3 Assessment of general embryonic pattering following global misexpression of competence factors. Plasmid vectors for hs:tfap2a or hs:gata3 were injected into wild-type embryos or Tg(hs;foxi1) transgenic embryos, as indicated across the top of the Figure. Embryos were heat shocked at 4.5 hpf, including the non-transgenic controls. A–F, expression of various markers at the indicated times: (A–C) neurectodermal markers sox19b, krox20 and fgf3 [56], [57], (D) Fgf-target gene erm, (E) epidermal marker p63 [46], [47] and (F) Bmp target gene sizzled [34]. Misexpression of competence factors does not block Bmp or Fgf signaling nor general features of axial patterning, though embryos appear partially dorsalized. (G) AO staining in the respective transgenic carriers. hs:gata3 showed reduced cell death while other transgenes alone or in combination resulted in slightly increased cell death compared to control embryos. (H) Lateral views of live embryos at 28 hpf. A–C, E, and G show dorsal views of embryos, D shows dorsolateral views, and F and insets in E show lateral views.