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Fig. 3 Expression of the gpr177 gene in zebrafish embryos. Expression of gpr177 mRNA was analyzed by whole mount in situ hybridization (A–I). A: Early somitogenesis, lateral view. B: 18 hpf, lateral view. C: 24 hpf, lateral view. D: 48 hpf, lateral view of head. E: 48 hpf, dorsal view of head. F: 48 hpf, lateral view of ear. G: 72 hpf, lateral view of head. H: 72 hpf, dorsal view of head. I: 72 hpf, lateral view of ear. All views are anterior to the right. Arrowheads indicate protrusions of semicircular canals. BA, branchial arches; T, tectum; HB, hindbrain, MHB, midbrain-hindbrain boundary; SC, spinal cord; TB, tail bud; VZ, ventricular zone. J: Western blot containing lysates from embryonic zebrafish between 1 and 10 hpf were probed using anti-GPR177 antibodies. Data are representative of four separate experiments.