Fig. 2

Fig. 2 Bbs8 and Vangl2 act on normal actin organisation and otolith formation. A-D) Visualisation of F-actin in the somite region of zebrafish embryos at 48 hpf by phalloidin. A) Control embryo. B) Injection of bbs8-MO caused undulation of actin filaments. C) In tri^-/- embryo the dorsal somites became broad and rectangular with an altered pattern of actin bundles, while the ventral somites were disorganised. D) Injection of bbs8-MO into tri^-/- background totally disrupted actin organisation. E-M) Observation of otoliths in zebrafish inner ear at 48 hpf. 1x, 2x, and 3x represent 1, 2 or 3 otoliths respectively. E) Control embryo with two otoliths. H, K) tri^-/- embryos showed varied number of otoliths (two or three). F, G, I, J, L, M) Injection of bbs8-MO into tri^-/- (G, J, M) or siblings (F, I, L) also resulted in varying numbers of otolith (one to three). Otoliths also appeared to be smaller (G, J, M) than normal. A-D) Lateral view, anterior to the left. A-D) Scale bar: 200 μM; E–M) Scale bar: 100 μM.