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Fig. S6 kctd15 RNA overexpression affects placode development. (A-L) Control uninjected embryos (A,C,E,G,I,K) and kctd15a RNA-injected embryos (B,D,F,H,J,L) were analyzed for the expression of placodal genes. kctd15a RNA-injected embryos show expansion of eya1 (B, 52/65) and six4.1 (D, 32/48) in the anterior preplacodal domain; similarly, lim3 (F, 35/40) and prl (H, 30/35) expression were expanded in the anterior pituitary placode. The otic placode, as revealed by the early marker foxi1, is unaffected (I, J), whereas the late marker cldna indicates a reduction in size in kctd15a RNA-injected embryos (L, 40/55) as compared with controls (K). Developmental stages are indicated in the lower right of each panel; arrowheads show lim3 expression; arrows show prl expression in the anterior pituitary. (A-D,I,J) Dorsal view, anterior to the top; (E-H,K,L) lateral view, anterior to the left. Scale bars: 200 μm in A-D; 150 μm in E-H; 100 μm in I,J; 300 μm in K,L.