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Fig. 9 Role of ER in calycosin-induced angiogenesis.
(A) Effects of ICI182, 780 on calycosin-induced HUVEC proliferation. HUVECs were pre-treated with ICI182, 780 (30 μM) before the addition of calycosin (100 μM). Data are expressed as percentage of vehicle control (100%) in mean±SEM (n = 3 independent experiments), *P<0.05 versus control, #P<0.05 versus calycosin. (B) Effect of ICI182, 780 on calycosin-induced activation of ERK1/2. Calycosin-stimulated phosphorylation of ERK1/2 was completely reversed by the absence of ICI182, 780 (30 μM). Expression of phospho-ERK1/2 and total-ERK1/2 was analyzed by western blotting and quantified by densitometry. The values indicate the relative densitometric units of the p-ERK1/2 bands with the density of the control band set arbitrarily at 1.0. Results are represented as mean±SEM. “cal” and “ICI” are the abbreviations of calycosin and ICI182, 780 respectively.