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Fig. 7 The effects of calycosin on HUVECs in vitro.
(A) Effects of calycosin on proliferation of HUVEC by XTT assay. HUVECs were seeded in 96-well plates and incubated with calycosin at different concentrations. Cell proliferation was assessed using XTT assay; (B) Tube formation of calycosin-treated HUVECs on Matrigel. HUVECs cultured on 3-dimensional Matrigel in treatment of calycosin (3 μM, 10 μM, 30 μM and 100 μM). Cells receiving 0.1% DMSO served as vehicle control. Number of branching points in different concentrations of calycosin-treated HUVECs was calculated by computer software (Metamorph). Results are expressed as percentage of control (100%) in mean±SEM (n≥3 independent experiments), *P<0.05 versus control.