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Fig. 2 The effects of calycosin on endothelial cells population in SIVs of Tg(fli1:nEGFP) zebrafish embryos.
Each green light point represents one endothelial cell (GFP+). (A) Control: embryo treated with 0.1% DMSO at 96 hpf. (B–D) Calycosin: embryo treated with 10, 30, 100 μM calycosin at 72 hpf for 24 h, leads to an increase in endothelial cells. (a–d) Enlarged SIV region (x4.5) of A–D respectively. (E) Calycosin increases the number of endothelial cells in the SIV region in a dose-dependent manner. Data are plotted as mean±SEM, (n = 3), **P<0.01, #P<0.001.
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