Fig. 6

Fig. 6 Behavioral anomalies of opa3^ZM/ZM mutants. (A) Image of one abnormally swimming opa3^ZM/ZM adult fish and three normal opa3^ZM/ZM siblings. (B) Increased buoyancy in opa3^ZM/ZM adult fish. Asterisk (^*) indicates the statistical significance of the increase (chi-square test, P=2.1e–7). (C) Exaggerated vertical turns by opa3^ZM/ZM adult fish. Each dot represents combined average of maximum ascent and descent angles observed for an individual fish. Horizontal lines show the overall average and vertical lines show the standard deviations. Red lines and data points are significantly different from black lines and data points (t-test, P=0.0055). (D) Reduced spontaneous scoot initiation by opa3^ZM/ZM larvae. Circles show the average scoot frequency for four groups of 25 opa3^+/+ larvae and triangles show the average scoot frequency for four groups of 25 opa3^ZM/ZM larvae, each measured on three consecutive days. A significant reduction was observed in opa3^ZM/ZM larvae (ANOVA test, F[1,9]=23.4, P=0.001).