ZFIN Image: Bai et al., 2010, Fig. 1

Image

Figure Caption

Fig. 1 Genetic Suppressor Screen in the mon Mutant

(A) Scheme of generating viable mon transgenic fish using a BAC transgene.

(B) Scheme of the suppressor screen. BAC transgenic fish are green fluorescent. In F1 generation, three groups of embryos were obtained: transgene homozygous (mon; Tg/Tg), transgene heterozygous (mon; Tg/+), and embryos with no transgene (mon). Only transgene heterozygous (in the red circle) were raised up to adults. Double in situ hybridization of GFP and βe3 globin was performed on F2 haploid embryos. Note the GFP staining on mon; Tg haploids (strong in the head and weak throughout the body) but not on haploids lacking the transgene. “sup” indicates a suppressor mutation.

(C) Morphology of the sunrise mutant at 48 hpf.

(D) In situ hybridization of βe3 globin at 22 hpf.

See also Figure S1.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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Reprinted from Cell, 142(1), Bai, X., Kim, J., Yang, Z., Jurynec, M.J., Akie, T.E., Lee, J., LeBlanc, J., Sessa, A., Jiang, H., DiBiase, A., Zhou, Y., Grunwald, D.J., Lin, S., Cantor, A.B., Orkin, S.H., and Zon, L.I., TIF1gamma controls erythroid cell fate by regulating transcription elongation, 133-143, Copyright (2010) with permission from Elsevier. Full text @ Cell