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Fig. 5 Expression patterns of zebrafish ghra and ghrb in adult tissues (A) and during embryonic development (B), studied by RT-PCR and Southern blotting. EF1α transcripts were amplified as control. PCR with no template were carried out as negative controls (-). The full-length (expected) and the shorter amplicons are indicated by arrows (A and B). Sizes of the amplicons are: 898, 532, and 700 bp for ghra and 701, 420, 471 and 650 bp for ghrb. (C) Positions of primer pairs used in RT-PCR and probes used in Southern blotting for ghra and ghrb cDNAs.
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Reprinted from Gene expression patterns : GEP, 10(4-5), Di Prinzio, C.M., Botta, P.E., Barriga, E.H., Ríos, E.A., Reyes, A.E., and Arranz, S.E., Growth hormone receptors in zebrafish (Danio rerio): adult and embryonic expression patterns, 214-225, Copyright (2010) with permission from Elsevier. Full text @ Gene Expr. Patterns