Fig. 7

Fig. 7 sym1 retains mesoderm induction and axis dorsalizing activity. At the one-cell stage embryos were injected with 150 pg of sym1 mRNA (Foxd3^sym1) (B, E, H, K, N) or 25 pg of wild-type foxd3mRNA (Foxd3^wt) (C, F, I, L, O) and analyzed at the shield stage (6 hpf) (A–L) or at 24 hpf (M–O). In situ hybridization at shield stage of uninjected (A, D, G, J), sym1-injected (B, E, H, K), and foxd3-injected (C, F, I, L) embryos showing expression of cyclops (A–C), goosecoid (D–F), chordin (G–I), and no tail (J–L). Views shown are dorsal (A–F, J–L) or animal with dorsal right (G–I). Uninjected (M), sym1-injected (N), and foxd3-injected (O) embryos at 24 hpf. Shown are live embryos (lateral views with anterior left).