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Fig. 4 Phenotypes of translation blocking morpholino (MO1) knockdown embryos.
(A–B)13-somite stage. (C–E) 24 hpf. (F–H) 48 hpf. (I–J) 72 hpf. (K–N) somite of mylz2-GFP transgenic zebrafish embryos at 48 hpf. (O–P) mylz2-GFP transgenic zebrafish embryos at 7dpf. A, C–D, F–G, I, K, M and O are Atoh8-MO treated embryos: A, C and F show severe abnormality, D, G, I, K, M and O show mild abnormality, and B, E, H, J, L, N and P are control-MO treated embryos. Atoh8-MO-treated embryos show shrunken eyes and head, a curved body axis with indistinct somite boundaries and yolk extension, accompanied by a U-shaped somite formation. In addition, the branchial arches are greatly reduced(O–P). In all panels but P, the dorsal view is up and the anterior is to the left. P shows the ventral view and the anterior to the left. Abbreviations: re, retina; sm, somite; ba, branchial arches.