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Fig. S2 Expression profile of ovo1 and efficacy of Ovo1 MOs. (A) RT-PCR for ovo1 (upper panel) at: 1.5-72 hours post-fertilization (hpf). ef1a (lower panel) is a loading control. (B,C) Live embryos injected with a construct carrying the Ovo1 MO binding site (Ovo1MO 5′UTR) fused to gfp (Ovo1MO 5′UTR:Gfp) showing Gfp expression (B) and loss with co-injection of the 5′UTR Ovo1MO (C).
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