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Fig. 4
Modulation of Tg(lhx1a:EGFP)pt303 expression by SB-431542.
(A-O) Shield stage Tg(lhx1a:EGFP)pt303 embryos, animal view. (A,D,G,J,M) Untreated control embryos. (B,E,H,K,N) Embryos treated with 0.8% DMSO. (C,F,I,L,O) Embryos treated with 800 μM SB-431542. (A-C) Tg(lhx1a:EGFP)pt303 expression. (D-F) In situ hybridization for lhx1a. (G-O) Fluorescent in situ hybridization. (G-I) egfp. (J-L) eve1. (M-O) Merged images.
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