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Fig. 4 Efficacy of pbx morpholinos. A: Efficiency of translation-blocking morpholinos (Pbx1MO, Pbx2MO, Pbx4MO) in blocking translation as shown by in vitro translation of corresponding mRNA alone, with standard control antisense morpholino (StdMO) or with morpholinos. B: Efficacy of splice-blocking morpholinos. RT-PCR shows decreased mRNA expressions level for embryos injected with splice-blocking morpholino (Pbx1-Ex2MO, Pbx2-Ex4MO, Pbx4-Ex3MO) as compared to their respective missense control (Pbx1-Ex2MM, Pbx2-Ex4MM, Pbx4-Ex3MM).
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