Fig. S2

Fig. S2 Point mutations in the small GTPase domain, and coiled-coil deletion disrupt cilia rescue activity. (A,B) Cilia in the pronephric duct (arrow) at 50 hpf as shown by anti-acetylated tubulin immunostaining in whole-mount embryos; side views at low magnification. Mut, sco^hi459 mutant sibling; wt, wild type. (C) 265.5 pg mRNA encoding alr13b alleles translationally fused to gfp were injected into hi459^+/-xhi459^+/- embryos at the one- to four-cell stage. Embryos were then scored at 2 dpf for body curvature, and stained with antibody against acetylated tubulin to evaluate the pronephric cilia phenotype. P-value is calculated from a chi-squared test with 1 degree of freedom. Results from a representative experiment are shown.