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Fig. 3 Gene products of arl13b are widely distributed. (A-C) In situ hybridization for arl13b on embryos at the 1-somite stage (A), the 8-somite stage (B) and 25 hpf (C). A and B are side views and C is a dorsal view, all with anterior to the left. In B, the transcript is enriched in the Kupffer′s vesicle (KV, arrow). In C, enrichment in the brain ventricle (red arrow) and the otic vesicle (black arrow) can be seen. (D-O) Arl13b is highly enriched in cilia in multiple organs as shown by whole-mount immunostaining of embryos at 2 dpf (D-L) and the 10-somite stage (M-O). The cilia marker anti-acetylated tubulin is shown in red, while anti-Sco is shown in green. (D-F) The pronephric duct (PD). (G-I) The spinal cord canal (SCC). (J-L) The olfactory placode (OLF). (M-O) The KV. Scale bars: 10 μm.