Fig. 2

Figures for Reischauer et al., 2009
Figure Caption

Fig. 2 Epidermal cells undergo EMT in pen/lgl2 mutant larvae.

Basal epidermal cells of wild type and pen/lgl2 mutant 5dpf stained using pan 1–8 Cytokeratin antibody (Ai - Bii) and E-cad antibody (C, D). Time lapse analysis of tg(ΔNp63::Gal4,UAS::GFP) labelled cells in wild-type (E) and pen/lgl2 mutant larvae (F) at 5dpf. Analysis of cell area in wild-type and pen/lgl2 mutant larvae (G). In contrast to wild-type larvae (Ai, Aii), in pen/lgl2 mutant larvae the cells appear spindle shaped with keratin accumulation around the nucleus (Bi, Bii). Furthermore, in wild-type basal epidermal cells (C), E-cad localizes to the cell membrane; cells exhibit perfect polygonal shapes. In pen/lgl2 mutant larvae (D) membrane localization of E-cad is strongly reduced with concomitant increase in the cytoplasmic fraction. Time-lapse analysis reveals (E, F) that there is no change in the shapes of wild-type epidermal cells (E). However, in pen/lgl2 mutants (F) the shape of epidermal cells dramatically changes over time, indicating their metastable cell fate. Cells develop lamellipodia like structures, a classic trait exhibited by mesenchymal cell types (see Videos S1, S2, S3, S4 in addition). As epidermal basal cells flatten and develop lamellipodia like cell protrusions in pen/lgl2, the cell area in apical view is increased in these larvae (G). However, average area of epidermal basal cells in these mutants is highly variable compared to wild types.

Figure Data
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