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Figure Caption
Fig. S2 Eight-point scoring system used to quantify retinal axon errors. Dorsal views of retinotectal projection of 5dpf isl2b:gfp wild type and ast larvae. (A) In wild-type embryos, retinal axons always pathfind to the tectum, and errors are almost never seen. (B) In ast-null mutants, eight classes of errors are commonly seen, including midline crossing at the habenular and posterior commissures, and projections to the left and right telencephalon, diencephalon and ventral hindbrain (stars). Counting the classes of errors made by misguided axons in each larva yields an error score between 0 and 8.
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