ZFIN Image: Hart et al., 2009, Fig. 3

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Figure Caption

Fig. 3 Taf3 selectively interacts with the C-terminal domain of Trf3 but not Tbp. A: Co-immunoprecipitation assays. Plasmids expressing Flag-tagged Taf3 and either myc-tagged Trf3, Tbp, or Trf3/Tbp chimeric protein were co-transfected into COS-7 cells. Taf3 was immunoprecipitated with an anti-Flag antibody, and the immunoprecipitates were analyzed for the presence of Trf3, Tbp, or Trf3/Tbp chimera by immunoblotting with an anti-myc antibody. B: Co-immunoprecipitation assays as described in A using HA-tagged TAF1. C: Schematic diagram of the zebrafish Trf3 protein, showing the substitution mutations. D: Co-immunoprecipitation assays. Plasmids expressing Flag-tagged Taf3 and either myc-tagged Tbp, wild-type Trf3, or Trf3 mutant were co-transfected into COS-7 cells. Tbp or Trf3 was immunoprecipitated with an anti-myc antibody, and the immunoprecipitates were analyzed for the presence of Taf3 by immunoblotting with an anti-Flag antibody.

Acknowledgments

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