PUBLICATION

Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis

Authors
Hart, D.O., Santra, M.K., Raha, T., and Green, M.R.
ID
ZDB-PUB-090929-8
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(10): 2540-2549 (Journal)
Registered Authors
Keywords
Taf3, Trf3, zebrafish, hematopoiesis, embryogenesis
MeSH Terms
  • TATA-Binding Protein Associated Factors/genetics
  • TATA-Binding Protein Associated Factors/metabolism*
  • Cell Line
  • Embryonic Development/physiology*
  • Hematopoiesis/physiology*
  • Embryo, Mammalian/cytology
  • Embryo, Mammalian/metabolism
  • Promoter Regions, Genetic
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Gene Expression Regulation, Developmental
  • Zebrafish*/embryology
  • Zebrafish*/physiology
  • Animals
  • Humans
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Transcription, Genetic
  • Molecular Sequence Data
  • TATA Box Binding Protein-Like Proteins/genetics
  • TATA Box Binding Protein-Like Proteins/metabolism*
  • Mice
  • Amino Acid Sequence
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 27)
PubMed
19777587 Full text @ Dev. Dyn.
Abstract
In zebrafish, TATA-box-binding protein (TBP)-related factor 3, Trf3, is required for early development and initiation of hematopoiesis, and functions by promoting expression of a single target gene, mespa. Recent studies have shown that in murine muscle cells, TRF3 interacts with the TBP-associated factor TAF3. Here we investigate the role of Taf3 in zebrafish embryogenesis. We find that like Trf3-depleted zebrafish embryos, Taf3-depleted embryos exhibit multiple developmental defects and fail to undergo hematopoiesis. Both Trf3 and Taf3 are selectively bound to the mespa promoter and are required for mespa expression. Significantly, Taf3 interacts with Trf3 but not Tbp, and a Trf3 mutant that disrupts this interaction fails to support mespa transcription, early development, and hematopoiesis. Thus, a selective interaction between Trf3 and Taf3 is required for early zebrafish development and initiation of hematopoiesis. Finally, we provide evidence that TRF3 and TAF3 are also required for hematopoiesis initiation in the mouse.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sd2TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    cdx4MO3-cdx4MRPHLNO
    taf3MO1-taf3MRPHLNO
    tbpl2MO1-tbpl2MRPHLNO
    1 - 3 of 3
    Show
    Fish
    Fish
    sd2Tg
    sd2Tg + MO1-taf3
    WT
    WT + MO1-taf3
    WT + MO1-tbpl2
    WT + MO3-cdx4
    1 - 6 of 6
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    DsRedEFGDsRed
    1 - 1 of 1
    Show
    Mapping
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    Citation
    Hart, D.O., Santra, M.K., Raha, T., and Green, M.R. (2009) Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(10):2540-2549.