Image
|
Figure Caption
Fig. S1 Specificity of sdc2 MO. Embryos were injected at the single-cell stage with sdc2 splice-blocking morpholino (SB MO) at the indicated concentrations. RNAs were extracted and analyzed by RT-PCR. An increased concentration of SB MO correlated with an increased accumulation of intron 2 (929 bp) in sdc2 RNA and a decreased accumulation of sdc2 spliced mRNA (145 bp). Diagram indicates the position of SB MO (red bar) and RT-PCR primers (blue arrows). Retention of intron 2 is predicted to lead to premature translation termination.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Development