IMAGE

Fig. 3

ID
ZDB-IMAGE-090817-18
Genes
Antibodies
Source
Figures for Moro et al., 2009
Image
Figure Caption

Fig. 3 In vitro and in vivo analysis of chimeric TKGFP protein expression. (A) Fluorescence microscopy image of NIH 3T3 murine fibroblasts transfected with the plasmid pCS2-TKGFP. Cells successfully transfected display a highly intense nuclear fluorescent signal. Fluorescent cells were visualized and photographed 24 h after transfection. (B) Fluorescent microscopy image of a 72 hpf stable Tg(-1.2ins:TKGFP) zebrafish. The green fluorescent beta cell nuclei of the islet are indicated by an arrow. (C) Tg(-1.2ins:TKGFP) embryos exhibit a nuclear specific fluorescent signal in beta cells. Confocal image of a representative immunofluorescence with anti-insulin and anti-GFP antibodies on 20 dpf Tg(-1.2ins:TKGFP) larvae. A representative islet with green nuclei and insulin staining (red) is depicted. (D–F) Confocal microscopy images of GFP expression in living Tg(-1.2ins:TKGFP) embryos at different developmental stages. Beta cells express the GFP in the nucleus with a strong specific signal. Each image is a superimposed projection of a 0.1 mm scanning. (D) 24 hpf; (E) 48 hpf; (F) 6 days.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 332(2), Moro, E., Gnügge, L., Braghetta, P., Bortolussi, M., and Argenton, F., Analysis of beta cell proliferation dynamics in zebrafish, 299-308, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.