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Fig. S6 Normal proliferation of clint1 morphant cells in a wild-type environment and unaffected Pkcζ localization in clint1 mutants. (A-B′) Cells from uninjected (A,B) or clint1-ex1 MO-injected (A′,B′) Tg(β-actin:hras-eGFP) embryos were transplanted to the ventral ectoderm of non-transgenic wild-type embryos. (C) Quantification of GFP-positive cells in unlabeled recipients at 2 and 3 dpf. (D,E) Transverse sections of wild-type (D) and clint1 mutant (E) embryos immunolabeled for Pkcζ (green) and p63 (red) and with DAPI (blue) at 48 hpf.
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