ZFIN Image: Dong et al., 2009, Fig. 7

Image

Figure Caption

Fig. 7 Tissue-specific knockdown of endogenous gata-1 expression.

(A) The expression of DsRed fluorescence and transcripts recapitulates the endogenous lmo2 expression pattern in the Tg(zlmo2:mir-shRNA^gata-1-actin-DsRed-BGH) ^{line 3}. Note that stronger expression of DsRed transcripts can be observed in the PBI region than in the ICM region. ICM: intermediate cell mass; PBI: posterior blood island. (B) WISH analysis of gata-1 expression in the DsRed^- and DsRed⁺ F1 siblings at 22 hpf. Red star denotes the gata-1 staining in the developing kidney. (C) WISH analysis of pu.1 expression in the DsRed^- and DsRed⁺ F1 siblings at 22 hpf. Note that the pu.1 staining is massively increased (arrow). All embryos are dorsal view with head to the left.

Acknowledgments

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