ZFIN Image: Aanstad et al., 2009, Fig. S8

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Figure Caption

Fig. S8 Convergence-extension and dorsal specification defects in cilia knock-down embryos
In situ hybridization for convergence-extension (A-D) and dorsal specification (E-H) marker genes at gastrula stages. Injection of either dnKif3b mRNA, qilin MO1 or qilin MO2 caused convergence-extension defects, suggesting perturbation of Wnt/Planar Cell Polarity signaling, and expansion of chd but not gsc expression, suggesting an upregulation of canonical Wnt signaling. Shown are controls and embryos injected with qilin MO1. All injections gave similar phenotypes. (A-D) in situ hybridization for ntl (A-B) and papc (C-D) at 10 hpf, showing convergence-extension defects. (E-H) in situ hybridization for chd (E-F) and gsc (G-H) at 6hpf. chd expression was expanded in qilin MO1 injected embryos (F) compared to controls (E), whereas gsc expression (H) was comparable to controls (G).

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