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Fig. S1

ID: ZDB-IMAGE-090710-72
Publication: Aanstad et al., 2009 - The Extracellular Domain of Smoothened Regulates Ciliary Localization and Is Required for High-Level Hh Signaling
All Figures: Figures for Aanstad et al., 2009

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Figure Caption

Fig. S1 Purmorphamine stabilizes, but cannot induce full activity of SmoC151Y.
(A-B) Western blots showing that purmorphamine treatment increased SmoC151Y levels from less than that of wt Smo to levels similar to, or above, wt Smo in untreated cells. (A) Zebrafish embryos from a smo^hi1640+/- heterozygote incross were injected with mRNA encoding Myc-tagged wt Smo or SmoC151Y, and treated with DMSO or purmorphamine. (B) Smo^-/- MEFs were transfected with wt Smo or SmoC151Y and treated with DMSO or purmorphamine as indicated. (C) Whereas wt Smo caused a robust activation of the Gli-luciferase reporter in response to either Shh or purmorphamine treatment, SmoC151Y did not display full activity in Smo^-/- MEFs.

Acknowledgments

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