ZFIN Image: Rothschild et al., 2009, Fig. 7

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Figure Caption

Fig. 7 Tbx5 morphants and mutants decrease CaMK-II levels. Camk2b2 in situ hybridization of 36 hpf embryos injected with 5 ng tbx4 MO (a, b) or 5 ng tbx5 MO (c, d) or uninjected hst siblings (e, f) and hst mutants (g, h) as determined phenotypically. Dorsal views (a, c, e, g) mark fins with arrow heads and head on views (b, d, f, h) mark atrium and ventricle. Scale bar = 0.2 mm. (i) CaMK-II activity was assessed at 72 hpf in at least 5 embryos per condition by CaMK-II peptide assay in the presence of Ca²⁺/CaM. Conditions include uninjected embryos (Uninj), camk2b2 MO A and MO B embryos at 2 ng (b2-A and b2-B), embryos co-injected with 2 ng camk2b2 MO B and 40 pg wild type δ_C CaMK-II cDNA (b2 MO + CaMK), tbx4 and tbx5 morphants (5 ng), hst embryos with (-/-) the hst phenotype (hst mut.) and siblings without (+/-; +/+) the hst phenotype (hst norm.).

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Acknowledgments

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Reprinted from Developmental Biology, 330(1), Rothschild, S.C., Easley, C.A. 4th, Francescatto, L., Lister, J.A., Garrity, D.M., and Tombes, R.M., Tbx5-mediated expression of Ca(2+)/calmodulin-dependent protein kinase II is necessary for zebrafish cardiac and pectoral fin morphogenesis, 175-184, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.