ZFIN Image: Phelps et al., 2009, Fig. S6

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Figure Caption

Fig. S6 ctbp1 Knockdown Does Not Affect β-Catenin Levels
(A) apc^mcr zebrafish embryos were injected at the one-cell stage with ctbp1 morpholino 1. At 48 hpf, whole embryo protein was harvested and subjected to western blot analysis for ctbp1 (top), β-catenin (middle) and vinculin (bottom). (B) apc^mcr zebrafish embryos were injected at the one-cell stage with ctbp1 morpholino 2. At 72 hpf, the embryos were fixed and subjected to in situ hybridization for i-fabp, NaPi, hoxa13a and evx1. (C) At 48 hpf, whole embryo protein was harvested from embryos injected as in (B) and subjected to western blot analysis for ctbp1 (top) and vinculin (bottom). The images are representative of three individual experiments.

Acknowledgments

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Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell