ZFIN Image: Phelps et al., 2009, Fig. S1

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Figure Caption

Fig. S1 KRAS Promotes Intestinal Cell Proliferation and Activation of β- Catenin following Loss of apc
(A) apc^mcr zebrafish embryos were injected at the one cell stage with KRAS mRNA. The embryos were fixed at 72 hpf, sectioned and stained using hematoxylin and eosin (H&E). Shown are representative sagital sections. (B) Zebrafish embryos harboring an integrated β-catenin TOPdGFP reporter were injected with KRAS^G12D mRNA, apc morpholino or both. The embryos were fixed at 72 hpf and subjected to whole mount in situ hybridization using GFP as a probe. (C) mRNA was harvested from 72hpf embryos injected as in (B). PCR was performed for GAPDH and unspliced apc. All images represent at least three independent experiments. (Scale Bar: 10μm).

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Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell