ZFIN Image: Phelps et al., 2009, Fig. 4

Image

Figure Caption

Fig. 4 KRAS/RAF1 Regulation of β-Catenin Requires the Activity of RAC1

(A and B) SW-480 cells transfected with vehicle or myc-tagged ([A], S191A or S605A) or flag-tagged ([B], S552A or S675A) β-catenin mutants were stained for DNA (magenta) and α-myc ([A], green) or α-flag ([B], green).

(C) SW-480 cells were transfected with RAC1-directed siRNA, DN-RAC1, and DN-Cdc42 or treated with the RAC1-specific inhibitor NSC23766 and stained for DNA (magenta) and β-catenin (green).

(D) SW-480 cells were subjected to an MTT assay (^*p < 0.05 versus DMSO; error bars, SEM).

(E) SW-480 cells were transfected with vehicle, KRAS^G12V-targeted siRNA, RAF1 siRNA, or DN-MEK1 and subjected to a RAC1 activity assay. The western blot was probed for RAC1 (top). Control lysates were probed for total RAC1 (bottom).

(F) SW-480 cells treated as above were stained for phospho-cJun.

(G) Human 293 cells transfected with constitutively active RAC1 and treated with PGE₂ or DMSO were stained for DNA (magenta) and β-catenin (green).

All images were captured using the same exposure and are representative of at least three independent experiments. Scale bar, 10 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell