ZFIN Image: Phelps et al., 2009, Fig. 2

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Figure Caption

Fig. 2 KRAS and RAF1 Are Necessary for Nuclear Localization of β-Catenin and Intestinal Cell Proliferation following Loss of APC

(A) apc^mcr zebrafish embryos injected with constitutively active RAF1 or MEK1. The 72 hpf embryos were stained for hematoxylin and eosin (H&E) staining (top), DNA (magenta, middle and bottom), and either PCNA (middle, green) or β-catenin (bottom, green).

(B) SW-480 cells transfected with control, KRAS^G12V, RAF1-directed siRNA, or DN-MEK1 constructs were stained for DNA (magenta) and β-catenin (green).

(C) RNA was harvested and subjected to rt-PCR for Axin2. Error bars, SEM.

(D) SW-480 cells transfected with KRAS^G12V-specific siRNA were cotransfected with constitutively active KRAS, RAF1, or MEK1 and stained for DNA (magenta) and β-catenin (green).

Overlapping expression is shown in white. All images were captured using the same exposure and represent three independent experiments. Scale bar, 10 μm.

Acknowledgments

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Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell