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Fig. 2

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ZDB-IMAGE-090527-19
Source
Figures for Phelps et al., 2009
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Figure Caption

Fig. 2 KRAS and RAF1 Are Necessary for Nuclear Localization of β-Catenin and Intestinal Cell Proliferation following Loss of APC

(A) apcmcr zebrafish embryos injected with constitutively active RAF1 or MEK1. The 72 hpf embryos were stained for hematoxylin and eosin (H&E) staining (top), DNA (magenta, middle and bottom), and either PCNA (middle, green) or β-catenin (bottom, green).

(B) SW-480 cells transfected with control, KRASG12V, RAF1-directed siRNA, or DN-MEK1 constructs were stained for DNA (magenta) and β-catenin (green).

(C) RNA was harvested and subjected to rt-PCR for Axin2. Error bars, SEM.

(D) SW-480 cells transfected with KRASG12V-specific siRNA were cotransfected with constitutively active KRAS, RAF1, or MEK1 and stained for DNA (magenta) and β-catenin (green).

Overlapping expression is shown in white. All images were captured using the same exposure and represent three independent experiments. Scale bar, 10 μm.

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Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell