Fig. 3

Fig. 3 Expression patterns of zebrafish slit3 mRNA. (A) Expression of slit3 mRNA at 70% epiboly in the axial mesoderm (am, white arrow). Dorsal view. (B, C) Expression of slit3 mRNA in 10-h embryos. Dorsal views of the rostral (B) and caudal (C) parts of the embryo. slit3 mRNA is expressed in the polster (p) as well as in axial mesoderm cells (white arrow). (D) Expression of slit3 mRNA in 26-h embryo. slit3 mRNA is expressed in the motor neurons in the ventral spinal cord (arrows). The lower inset shows the close-up view of the motor neurons. (E–G) Expression of slit3 mRNA in the head region of 48-h embryos. E, lateral view; F, a sagittal section; G, dorsal view. slit3 mRNA is expressed in the cranial motor neurons (arrowheads) and the floor plate cells (fp) in the hindbrain. (H) Expression of Islet-1 mRNA in the head region of 48-h embryos. Islet-1 mRNA is also expressed in the cranial motor neurons (arrowheads). III, IV, Va, Vp, VII/VIII, X, oculomotor, trochlear, anterior trigeminal, posterior trigeminal, facial/octavolateralis, and vagus motor neurons, respectively. (I–L) Expression of slit3 mRNA in various parts of 48-h embryo. slit3 mRNA is expressed in the pectoral fin bud (I), the pharyngeal arches (m, h, b in J), spinal motor neurons, and the roof plate (arrows and white arrowhead in K, respectively) and the lens (l in L). I, lateral view; J, a parasagittal section; K, a cross section of the anterior spinal cord; L, a frontal section of the head. m, h, and b, the mandibular arch, the hyoid arch, and the posterior set of branchial arches, respectively; e, eye; ov, otic vesicle; s, somites; n, notochord. Bars, 200 μm (A–D, E–H, L), 100 μm (I–K).