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Fig. 3 gsc recruits unlabeled cells to secondary axes. (A-K) Zebrafish embryos injected with gsc RNA. (A-C) Lateral views, (A) bud; (B,C) 1 dpf. (D,E) Animal views; (F-K) dorsal views, 1 dpf. (A,B,F,I) DIC; (G,J) fluorescence; (C,H,K) merge. (A) Secondary notochord with somites (arrowheads) at bud. (B,C) Myotomes (arrowheads) with GFP-labeled notochord (dashed lines). (D,E) Shield stage gsc RNA-injected embryos stained for membrane-GFP (brown) and chd (purple), with ectopic chd (arrowheads). (F-H) Secondary neural tube with GFP-labeled (arrow) and unlabeled cells; arrowhead marks anterior GFP limit. (I-K) Low gsc RNA dose ventral clone produces partial secondary axis. (K) Arrows indicate unlabeled cells in neural tissue (right inset) and in myotomes (left inset). (L-O) Shield (arrowheads). (L,N) gfp RNA injected; (M,N) gsc RNA injected, with wnt8 (L,M) and chd (N,O) in situ probe. nt, neural tissue.