Fig. 4
Fig. 4 The effect of the twdrw685 mutation is specific to neuronal migration. (A-L) Commissural axons (arrowheads) labeled with zn-5 antibody at 36 hpf (A,B; lateral view, rostral towards the left), reticulospinal neurons retrogradely labeled with rhodamin-dextran (RDX) at 5 dpf (C,D; ventral view, rostral towards the top), Gad1/2-positive neurons at 48 hpf (E,F; cross-sections, dorsal towards the top), Lhx2/9-positive neurons at 48 hpf (G,H; cross-sections, dorsal towards the top), aPKC at 30 hpf (I,J; cross-sections, dorsal towards the top) and ZO-1 at 30 hpf (K,L; cross-sections, dorsal towards the top) are labeled (red) in the wild-type (A,C,E,G,I,K) and twdrw685 mutant (B,D,F,H,J,L) embryos.