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Figure Caption
Fig. S6 Nuclear β-catenin can be measured by comparison to DAPI staining. Optical section of a tailbud imaged laterally, stained with DAPI and antibody against β-catenin. The high levels of β-catenin at the cell membrane obscure other staining within the cell. Using the computer program ImageJ to define the area of the nuclei based on DAPI staining allows for specific exclusion of non-nuclear staining. The resulting composite clearly shows regions of active canonical Wnt signaling.
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Reprinted from Developmental Biology, 329(1), Row, R., and Kimelman, D., Bmp inhibition is necessary for post-gastrulation patterning and morphogenesis of the zebrafish tailbud, 55-63, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.